TLC of Oleuropein and other compounds in olive mill waste water lab write up
Methodology: For extracting OMW: 1. 2. 3. 4. 5. 6. Add 25 mg of OMW to a test tube Add 1.25 mL of H20 and 2.5 mL of ethyl acetate Place on rotary spinner for 5 minutes Centrifuge for 5 minutes. Retrieve ethyl acetate phase. Perform TLC analyses using revelator and analyse under fluorescence. For […]