Supplementary FigureS2 from Topacio et al (2019) Mol Cell 74:758- 770.
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Get it written →Below is the Supplementary FigureS2 from Topacio et al (2019) Mol Cell 74:758- 770. Please answer the following (a-f):
A Pan et al. 2001 B W&B 2004 W ACdk AH dk WT AHelix Q899P 32P-Rb variant A90 4xPro. coomassie 32P-Rb variant C coomassie WT T905A Q912A L901A 08A 4A R9 F897A M90 911 900A Helix D M T907A K915A rev. H NT Helix Helix mut. R908A L901/ F897A 2p-Rb variant R910 coomassie E 32P-Rb variant coomassie 5- +cyclinD1-Cdk4 G 3 +cyclinE1-Cdk2 W velocity (A.U./min) 2- velocity (A.U./min) left mut. + AHelix LXCXE cleft mut RxL mut. + AHeli WT RXL mut. Helix N. LXC UM substrate N- -Cdk4 RbWT (K =0.39 UM ) UM substrate 32P-Rb variant +cyclinE1 +cyclinD1 – RbHelix mut. (K, not calculated) – RbWT (K = 0.064 PM ) coomassie -Cdk2 – RibHelix mut. (K = 0.089 UM) 32P-Rb variant coomassie
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