Grossmont College Role of Plant Pigments in Photosynthesis Skill Check Worksheet
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Grossmont College Role of Plant Pigments in Photosynthesis Skill Check Worksheet
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Question Description
I’m trying to learn for my Biology class and I’m stuck. Can you help?
Question 1A: Based on the information in your pre-lab exercise, in the space below, rank order the pigment molecules that were discussed based on their polarity, starting with the most polar as number one.
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2.
3.
4.
Question 1B: Now, rank order the pigments based on their ability to dissolve in a non-polar solvent, listing the pigment that will dissolve the most as number one.
1.
2.
3.
4.
Question 1C: My hypothesis to answer the question “What pigments do you think you will find in a spinach leaf”?
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Question 1D: My prediction for the results based on my hypothesis
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Question 1E: How long did it take for the solvent front to reach the top of the paper?
Question 1F: Draw the results of the chromatography process, labeling any bands that you see and record the distance measurements of the bands and solvent front in the table below.
Calculate the Rf values for each of the bands and write the information next to the location of the band.
| Pigment or Solvent Name | Pigment Color | Distance Moved (cm) | Rf Value |
| Solvent Front | Clear | 12.1 | 1 |
| Pigment 1 | 11.9 | ||
| Pigment 2 | 5.5 | ||
| Pigment 3 | 4.6 | ||
| Pigment 4 | 4.2 |
Question 1G: What pigments did you find? How do you know? Do the data you obtained validate your hypothesis? Why or why not?
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Question 1H: Did the bands you see line up in the order that you would have predicted based on the information you were given in the pre-lab and in this exercise? If not, why not?
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