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August 8, 2024

Molecular Biology

1. How does the specific arrangement of amino acids in a protein sequence contribute to its
ability to bind to DNA molecules? Provide a detailed explanation of the key structural and
electrostatic factors that determine this binding affinity and give examples of amino acid
residues that are commonly involved in DNA-protein interactions

2. Outline the principles and all the steps involved in purifying proteins using ion exchange
chromatography. Describe how the charge properties of proteins and the ion exchange resin
facilitate the separation process. Make sure to explain the differences between cation
exchange and anion exchange chromatography.

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