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November 2, 2022

You are investigating the effects of TGF-beta on two breast cancer cell lines, X andY.

You are investigating the effects of TGF-beta on two breast cancer cell lines, X andY.

Since TGF-beta activates Smad transcription factors, you decided to overexpress Smad2 using a mammalian expression vector. You confirmed the overexpression of Smad2 by Western blot. When the cell lines were treated with TGF-beta, you observed that the X cell line stopped proliferation whereas the Y cell line looked elongated and motile.

Explain two differential phenotypes resulting from TGF-beta/Smad2 activation.

To confirm the differential phenotypes between X and Y cells you described in a, what marker(s) would you use on Western blot?

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