PRC Process Analysis Discussion

What challenges exist with designing multiplex PCR primers?
What are the advantages of a termal stable, hot-start DNA polymerase?
What are “stochastic effects” and why are they important to forensic DNA analysis?
What component of a PCR reaction is labeled with a fluorescent dye to enable detection of amplified PCR alleles?
Why is it important to separate pre-PCR and post-PCR processes? Explain what you would do if you won´t have the opportunity to separate these processes. Would your analysis be as effective? Justify your answers.
Why do you think the US DNA database (CODIS) recently increased the number of STRs including on the profiles uploaded on the database? Based on this, which STR kit should be used by forensic labs?
What are some approaches to dealing with the possibility of allele dropout due to PCR primer binding site mutations?
What steps are typically taken to confirm the presence of an off-ladder allele?

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