How enzymes work

https://learn.chm.msu.edu/vibl/vibl/Catalase/catalase_HTML5Canvas.html

Above is the link to the lab.

Worksheet_Catalase Test

Name:

Table 1. Results of catalase tests using two Gram positive cocci

Bacterial species

Bubbling

Catalase Test (Positive/Negative)

1

Streptococcus pyogenes

2

Staphylococcus aureus

Critical Thinking Questions:

1. What is the purpose of the catalase test?

2. What kind of bacteria produce catalase?

3. Why does a bacterium produce catalase?

4. What are the bubbles observed in a positive reaction?

5. If a bacterial strain is catalase positive, is it always an obligate (strict) aerobe? Explain.

6. If a bacterial strain is catalase negative, is it always an obligate (strict) anaerobe? Explain.

7. If a bacterial strain is an obligate (strict) aerobe but catalase negative, what enzyme does the bacterium may use to detoxify H2O2?

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