Cell Structure Lab

Cell Structure and Function Student Name Date 1 Pre-Lab Questions PLAN UP TO 3 HOURS FOR THIS LAB – if you want to split it up you can stop after Set up (30 minutes) or Activity 1 (approximately 1 hour) and complete Activity 2 on a second day (approximately 1.5 hours). Read the Background provided on the lab instructions, information from your textbook, and any information provided in the READINGS area of the module to help you answer these pre-lab questions: **Identify the major similarities and differences between prokaryotic and eukaryotic cells. **Watch this video and answer the following question: Video: Do Fish Drink Water? o Using what you have learned about osmosis, what would happen if you put a freshwater fish in saltwater? Activity 1 1. BEFORE YOU BEGIN: Read through the activity and collect your materials 2. Carefully read the safety notices for Potassium permanganate and methylene blue. Make Note of their properties below” a. **Potassium permanganate is an: b. **Methylene blue is an irritant of: 3. Follow all instructions in your instruction manual. 4. Be sure to TAKE A PHOTO of your petri dish and ruler at 0, 15, 30, 45, and 60 minutes. Insert these images after your data table. **Data Table 1 – Fill in the table below with your experimental data Time (min) Diameter, Potassium Permanganate (mm) 0 15 © 2017 Carolina Biological Supply Company Diameter, Methylene Blue (mm) 2 Time (min) Diameter, Potassium Permanganate (mm) Diameter, Methylene Blue (mm) 30 45 60 Insert the photo from Activity 1, step 9. The entire Petri dish should be visible, and each dye should be identified. 0 Min Photo: 15 Min Photo: 30 Min Photo: 45 Min Photo: 60 Min Photo: 1. **Create a graph of your data from Table 1. Using a graphing program, such as Microsoft Excel® and insert it below. Be sure that your graph is properly labeled: 2. Determine the rate of diffusion for each dye using the following formula: (𝑓𝑖𝑛𝑎𝑙 𝑑𝑖𝑎𝑚𝑒𝑡𝑒𝑟−𝑖𝑛𝑡𝑖𝑡𝑎𝑙 𝑑𝑖𝑎𝑚𝑒𝑡𝑒𝑟) 𝑅𝑎𝑡𝑒 𝑜𝑓 𝑑𝑖𝑓𝑓𝑢𝑠𝑖𝑜𝑛 (𝑚𝑚/𝑚𝑖𝑛) = 𝑡𝑖𝑚𝑒 (𝑚𝑖𝑛) a. **What is the rate of diffusion for Potassium Permanganate? b. **What is the rate of diffusion for Methylene Blue? 3. **The molecular weight of potassium permanganate is 158 g/mol. The molecular weight of methylene blue is 320 g/mol. Based on the information recorded in Data Table 1, what can you conclude about the relationship between molecular weight and diffusion? © 2017 Carolina Biological Supply Company 3 Activity 2 1. Read the procedure description thoroughly and become familiar with the kit materials and procedure steps prior to beginning. 2. Carefully follow all lab manual instructions. a. Dialyisis tubing must be rehydrated in clean tap water for at least 30m prior to your experiment. b. Watch this video: tips on opening your dialysis tubing 3. **Before you place your dialysis tubing into the experimental beakers, TAKE A PHOTO of all three pieces of filled dialysis tubing sitting in their weigh boats and insert it below. All three pieces of filled dialysis tubing should be visible: **Data Table 2 – Fill in the table below with your experimental data Treatment A Treatment B Treatment C Solution in dialysis tubing 20% sucrose 40% sucrose 20% sucrose Solution in cup 20% sucrose 20% sucrose 40% sucrose Initial volume (Vi) (mL) Final volume (Vf) (mL) Change in volume (Vf−Vi) (mL) © 2017 Carolina Biological Supply Company 4 Treatment A Treatment B Treatment C Percent change in volume (change in volume/Vi) ×100 Hypotonic, isotonic, or hypertonic 4. **Insert the photo(s) from Activity 2, step 20. All three pieces of dialysis tubing should be visible, and the concentration of the sucrose solution in which each was immersed should be noted: 5. **Data Table 2, what is the significance of a negative percent change in volume? 6. **Describe the similarities and differences between the dialysis bag and a cell membrane. Be specific. © 2017 Carolina Biological Supply Company

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