Distinguish between SNP and RFLPs: In the process of the restriction digest the SNP mutations in the CPY family are also responsible for creating or deleting already existing restriction sit
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The analysis of CYP2C9*3 detection after clopidogrel activation using CYP p450.
Abstract (100-250)
Aims
Brief results:
Brief conc:
Introduction (300-400 words)
Cytochrome p450 are a type of enzyme group which assists in drug activation and is an anticoagulant used to reduce the processes leading up to stent thrombosis in the
It is activated by.
Distinguish between SNP and RFLPs:
In the process of the restriction digest the SNP mutations in the CPY family are also responsible for creating or deleting already existing restriction sites. The addition and elimination are known when present in the enzyme DNA is called restriction fragment length polymorphisms.
Image of clopidogrel drug mechanism of action:
Research questions:
What is the function and purpose of the clopidogrel drug?
What is the purpose of a restriction digest?
What is the purpose for screening CYP29*3 variants on gel electrophoresis agarose gel? What are we trying to find out?
The separation of the different lanes and bands makes the results more distinguishable from each of the 10 patient samples to identify RFLPs for the contents experimented using the restriction digest of the kpn1 restriction site.
How will this information help us to understand the patients genetics and changes in their physical body regarding their diseases and drug pharm kinetics/pharmacology?
As with the DNA/protein coding of enzymes there can be mutations present called single nucleotide polymorphisms (SNPS) which result in a change in a single base pair of a gene coding for the specific enzyme. This can alternatively change the way the enzyme is initially supposed to work which therefore makes scientists consider other factors which this can influence such as the concentration of drug administered to patients with several CYP enzyme mutations in their DNA which codes for CYP inactivation. The enzyme functions denature and is degraded as This SNP polymorphism can lead to specific phenotypic changes such as motor impairment in the depending on the genotype (Homozygous wild type or homozygous mutant) of patients. This means Due to these genotypes careful consideration of drug intake needs to be taken into consideration as less drug can be concerted to active metabolite which means?
Results
Image of results from canvas:
Figure 1:
Description of gel electrophoresis
The 10 different lanes are used in the agarose gel electrophoresis showing the different patient diagnosis their diseases and the detection of the band numbers and levels across the gel per sample. This is indicative of
Describe the thickness or colour of the band. What does this show in terms of pharmacogenomics?
Discussion (500-700)
How do the results affect dosage for patients exhibiting certain mutations for CYP p450 family?
What does it mean for patient phenotype?
Discuss the impact of mutation on clopidogrel treatment and how this might affect anticoagulant therapy.
References (In Vancouver)
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