MCB2340C Module 01 Quiz Latest 2023

MCB2340C Section 04 General Microbiology
Module 01 Quiz
Question 1
Rod-shaped bacteria, regardless of their ability to form endospores, are referred to as ___________.
1.Cocci
2.Bacilli
3.Spirillum
4.Vibrioid
Question 2
Chemically, proteins can function as
1.Energy storage
2.Information storage
3.Enzymes
4.Pigments
Question 3
__________ includes the organisms yeast and mold.
1.Algae
2.Bacteria
3.Fungi
4.Viruses
Question 4
After you dry your hands with a paper towel, where should you dispose of the paper towel?
1.Garbage can
2.Biohazard container
3.Sharps container
4.Empty sink
Question 5
The aseptic technique involves:
1.Wearing the appropriate PPE to protect yourself, the organism, and the environment from contamination.
2.Using sterilized media and equipment to prevent contamination of the organism.
3.Making sure air currents are not present that could bring contaminants in or out of your vessels.
4.All answers are true.
Question 6
The scientific method involves:
1.Planning an experiment to test your hypothesis.
2.Analyzing the results and concluding whether your hypothesis was correct.
3.Making sure your biases do not overrule the data collected.
4.All answers are true.
Question 7
Which of the following industries use(s) microorganisms for critical steps?
1.Wine and beer
2.Cheese and yogurt
3.Wastewater
4.All answers are true
Question 8
Which of the following have either DNA or RNA but not both?
1.Archaea
2.Bacteria
3.Prions
4.Viruses
Question 9
Viruses are easily viewed with the brightfield microscope using the 100X objective lens and immersion oil.
1. True
2.False
Question 10
Thin, flexible spiral bacteria are called:
1.Cocci
2.Spirillum
3.Vibrio
4.Spirochete
Question 11
Fungi and some protozoa produce a compound called _____ which strengthens the cell membranes
1.cholesterol
2.ergosterol
3.hopanoids
4.estrogen
Question 12
The sharps container should be used to dispose of which of the following items?
1.Used paper towels
2.Used gloves
3.Bacterial culture plates
4.Used microscope slides
Question 13
Some bacteria strengthen the cell membrane by incorporating various ___________ compounds in it.
1.Cholesterol
2.Ergosterol
3.Hopanoid
4.Estrogen
Question 14
The aseptic technique involves:
1.Protecting yourself and others from contamination
2.Protecting your pure culture from contamination
3.Protecting your environment from contamination
4.All answers are true
Question 15
Cocci arranged in squares of four in irregular clusters are called:
1.Diplococci
2.Streptococci
3.Tetrads
4.Sarcinae
Question 16
Animal, plant, and algal cells produce a compound called __________ that strengthens the cell membranes.
1.Cholesterol
2.Ergosterol
3.Hopanoid
4.Estrogen
Question 17
Multicellular, parasitic organisms that spend most of their time in a mammalian host are called ________.
1.Protozoans
2.Fungi
3.Helminths
4.Algae
Question 18
The term _______________ describes the cloudy growth one sees in a broth after inoculation with a culture and overnight incubation.
1.Precipitate
2.Pellicle
3.Sediment
4.Turbidity
Question 19
Chemically, lipids can function as
1.Transport molecules
2.Hormones
3.Receptors
4.Structural components
Question 20
Biosafety level 3 involves
1.Microbes that are dangerous and exotic and often cause fatal disease
2.Organisms such as Mycobacterium tuberculosis
3.Protective suits with their own air supply and positive pressure relative to the outside
4.All answers are true
Question 21
Which type of media provides the best option for obtaining a culture with isolated bacterial colonies?
1.Sim tubes
2.Nutrient agar plates
3.Nutrient broth tubes
4.Semi-solid agar tubes
Question 22
The person considered to be the “father of microbiology” and who was the first to observe live bacteria and protozoans was:
1.Antony van Leeuwenhoek
2.Robert Hooke
3.Louis Pasteur
4.Joseph Lister
Question 23
Eukaryotic microorganisms include fungi, protozoans and algae.
1. True
2. False
Question 24
When first using a brightfield microscope to view a specimen, which objective lens should be the first you use?
1.100X
2.40X
3.10X
4.4X (scanning objective)
Question 25
The Centers for Disease Control guidelines suggest that you should scrub with soap for a minimum of _________ seconds
1.10
2.15
3.5
4.20
Question 26
The aseptic transfer from one glass
tube into another should be accomplished by doing which of the following actions to the tubes?
1.Hold the mouth of one tube in the flame while the inoculating tool is inserted for removal of organisms.
2.Remove the cap of one tube and place it on the table away from the flame.
3.Hold the bottom of the tube in the flame for 10 seconds before transferring the culture.
4.The mouth is passed through the flame right when opened and just before closing.
Question 27
You notice that your lab partner has the following items on the lab bench. Which would you suggest they remove before beginning the lab activity?
1.Notebook
2.Pencil
3.Starbuck’s coffee
4.Safety goggles
Question 28
Starch is an example of a __________
1.Protein
2.Carbohydrate
3.Lipid
4.Nucleic acid
Question 29
When using a microscope, the ability to distinguish fine detail or discriminate between two very closely spaced objects is called:
1.Microscopy.
2.Resolution.
3.Clarification.
4.Magnification.
Question 30
Which item is used to support the growth of a microbial population?
1.Inoculating loops
2.Inoculating needle
3.Sterile water
4.Culture medium
Question 31
Into what type of container should used microbiology gloves be disposed of?
1.Garbage can
2.Biohazard container
3.Sharps container
4.Empty sink
Question 32
The scientific method doesn’t involve:
1.Asking a question.
2.Forming a hypothesis.
3.Planning an experiment.
4.Making the data fit your hypothesis no matter what.
Question 33
Beadlike chains of cocci formed after cell division along a single axis are called:
1.Diplococci
2.Streptococci
3.Tetrads
4.Staphylococci
Question 34
If you look at an agar plate and you see a fuzzy colony apart from your original bacterial streak that has colonies that appear round and glossy, this is called contamination.
1. True
2. False
Question 35
Chitin is an example of a __________
1.Protein
2.Lipid
3.Carbohydrate
4.Nucleic acid
Module 01 Lab Quiz
Question 1
All these terms categorize microorganisms based on their temperature preference EXCEPT
Obligate halophile
Psychrophile
Mesophile
Psychrotroph
Question 2
If the heat-fixation step is omitted in a simple stain, what will happen?
The dye will not be taken up by the cells
The staining process will destroy the cells
The cells can wash off the slide
The cell morphology will change
Question 3
Gram-negative cell walls contain a thin layer of peptidoglycan, whereas Gram-positive cell walls contain a thick layer of peptidoglycan.
True
False
Question 4
All the following organisms are expected to grow within an anaerobe jar EXCEPT
Obligate anaerobe
Aerotolerant anaerobe
Obligate aerobe
Facultative anaerobe
Question 5
If a bacterium were placed in a hypertonic solution prepared by the addition of sucrose, which of the following would happen to the bacterium?
The bacterial cell would swell but not lyse because of the presence of the cell wall
The bacterial cell would not change
The bacterial cell would undergo osmotic lysis
The bacterial cell would undergo plasmolysis
Question 6
All the following are environmental factors that affect microbial growth EXCEPT
Temperature
Osmotic pressure
pH
Cell wall type
Question 7
A ___________ is used to remove the primary stain from the bacterial cell wall in a differential staining technique.
Mordant
Decolorizer
Secondary stain
Counterstain
Question 8
In the acid-fast stain, non-acid-fast bacteria appear blue under the microscope.
True
False
Question 9
An acidophile is an organism that has an optimal pH for growth below 5.5.
True
False
Question 10
All microorganisms use oxygen in their metabolism.
True
False
Question 11
The environmental conditions on human skin have NaCl levels slightly above that of most bacterial cells. What category of organism would most likely be part of the normal skin microflora?
A halotolerant organism
An obligate halophile
An obligate anaerobe
A psychrophile
Question 12
Capsule staining techniques stain the background and the vegetative cell of an incapsulated bacterium because the capsule doesn’t react with most stains.
True
False
Question 13
All these terms categorize microorganisms based on their temperature preference EXCEPT:
Mesophile
Psychrophile
Psychrotroph
Chemoautotroph
Question 14
All the following are found in most prokaryotic cells EXCEPT:
cell membrane
cytoplasm
cell wall
Nucleus
Question 15
A ____________ is used in the Gram stain procedure to complex with the dye and trap it within the cell of a bacterium with a thick peptidoglycan cell wall?
Mordant
Decolorizer
Counterstain
Primary stain
Question 16
Gram positive cells appear purple after Gram staining whereas Gram negative cells appear pink.
True
False
Question 17
___________________ is used to stain the endospore in the endospore staining procedure.
Methylene blue
Crystal violet
Malachite green
Safranin
Question 18
Heat-fixation is performed in slide preparation in order to _________________________________.
Fix the morphology of the organisms on the slide
Encourage bacterial growth on the slide
Inactivate and adhere microorganism placed on the slide
Protect the bacteria by hardening them to prevent destruction by the staining procedure
Question 19
Heating or steaming a fixed smear with the primary stain, malachite green, enhances the penetration of the stain into this structure.
Capsule
Cell wall
Chloroplast
Endospore
Question 20
A hypotonic solution is one with a solute concentration greater than the concentration which exists within the cell.
True
False
Question 21
If a bacterium were placed in a hypotonic solution prepared by the addition of sucrose, which of the following would happen to the bacterium?
The bacterial cell would swell but not lyse because of the presence of a cell wall
The bacterial cell would not change
The bacterial cells will undergo osmotic lysis
The bacterial cells will undergo plasmolysis
Question 22
The simple stain technique provides enough information about a microbe to identify its species reliably:
True
False
Question 23
As long as the normal enzymatic function is not compromised, a microorganism will grow across the range of pH.
True
False
Question 24
Organisms that require a lower level of molecular oxygen for their metabolism than what is typically found in the atmosphere, are called __________________?
Microaerophiles
Obligate aerobes
Facultative anaerobes
Aerotolerant anaerobes
Question 25
Where would you expect an obligate aerobe to grow in a fluid thioglycollate media tube?
The upper part of the tube
The lower part of the tube
Throughout the tube
The middle part of the tube

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