I am mostly focused on parts (b) and (d), which I typed out. For (b), I can tell the first pair of graphs is a sequential reaction (when Cysteine is saturated), but it appears ping
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Get it written →I am mostly focused on parts (b) and (d), which I typed out. For (b), I can tell the first pair of graphs is a sequential reaction (when Cysteine is saturated), but it appears ping-pong for the others because the slopes are roughly parallel. I know sequential has to reactants binding (so I guess that would be ATP and GlcN), and I suppose that a reaction occurs there and then maybe Cysteine binds to react with an intermediate?
But then again, if Cysteine is saturated, I don’t know if that makes sense, because Cysteine should be able to bind.
Also, how do we know ATP binds first (part d)? Part (e) also confuses me, because ATP has the highest K_m, so the lowest binding affinity, but ATP is saturating the enzyme. It seems like there would be less binding for that.
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