EcoRI restriction enzyme is supplied at the concentration of 20U/ul. What volume of this enzyme would you need to put in a 100 ul reaction that contains 50 ug of pML104 plasmid to
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Get it written →EcoRI restriction enzyme is supplied at the concentration of 20U/ul. What volume of this enzyme would you need to put in a 100 ul reaction that contains 50 ug of pML104 plasmid to achieve a complete digest? You intend to incubate the reaction for 1 hr at 370C.
1 uL
2.5 uL
0.4 uL
2 uL
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