If the product from an upstream process produced using E. coli is intracellularly aggregated, describe what type of technologies and equipment would you use to harvest and recover the product for purification?

If the product from an upstream process produced using E. coli is intracellularly aggregated, describe what type of technologies and equipment would you use to harvest and recover the product for purification? Provide operating parameters for the selected equipment and answer questions:

How is this parameter being controlled? i.e. what variables caused this parameter to change?

What would happen if this parameter is out-of-control? Why do we monitor it?

Make a list of vendors and their websites who manufacture the equipment used to cause cell lysis and clarification for use in large scale commercial or clinical manufacturing. Make comments on the equipment features or specs (size, flow rates, etc.). I expect at least three pieces of equipment.

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