Why can you store your Unknown for more than one week in the refrigerator? (Check all that apply)
Question 1 What is the purpose of this lab exercise? (Check all that apply)
A. To make Simple stain slides
B. To learn the Gram Stain
C. Learn to prepare Negative stains
D. Learn to use an Electron Microscope
E. To identify an unknown organism
Question 2Why do you have to put your Unknown in fresh media each week? (Check all that apply)
A. The prep room has a lot of media prepared and it needs to be used up by the end of the semester.
B. You are very bored, have lots of time on your hands, and this is excellent “busy work”.
C. Every week there is metabolic waste accumulation that will kill your Unknown.
D. Every week nutrients found in the media are completely utilized.
E. There is no good reason
Question 3Why can you store your Unknown for more than one week in the refrigerator? (Check all that apply)
A. The cold temperatures speed up metabolic reactions
B. Every week there is metabolic waste accumulation that will help your Unknown thrive
C. The cold temperatures slow down metabolic reactions.
D. Microbes can’t live in the refrigerator.
E. There is no good reason.
Question 4What is a Dichotomous Key? (Check all that apply)
A. Identification is based on successive questions
B. The flow chart is in the shape of a key
C. No one knows, but the name sounds very cool and science-like.
D. After each question is answered, you are led to another question until the organism is identified
E. There are two possible answers in each step.
Question 5 After studying the Dichotomous Key, what do you think would be the first test you would want to perform on your Unknown?
Capsule stain
Simple stain
Acid Fast stain
There is no need to stain the Unknown organism to facilitate its identification.
Negative stain
Gram stain
Endospore stain
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