You have three strains of MHC incompatible mice, A, B and C. You raise antisera A anti-B. You make the following findings

You have three strains of MHC incompatible mice, A, B and C. You raise antisera A anti-B. You make the following findings:

A anti-B has antibodies against cells from B but not A strain mice, and also against cells of strains C and D.

A anti-B, absorbed with cells of strain D, still has antibodies specific for cells of strain C but not of D. We denote antibodies that react with D as recognizing epitope(s) 1.

A anti-B absorbed with D and C still lyses B. We denote the antibodies recognizing epitope(s) on C but not D as epitope 2. We denote the epitope(s) recognized by A anti-B, present on cells of B but not on cells of strains C and D, as epitope 3. We conclude B has epitopes 1, 2 and 3; C has epitope 2 and D has epitope 1. How would you determine if cells from strain C bear epitope 1?

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