Treatment on Pathogenesis
Treatment on Pathogenesis
Bacteria play a vital role in life on Earth, inhabiting soil, water, plants, and animals.
For example, bacteria in the soil break down organic materials, recycling nutrients back to the environment. Other bacteria live in and on our bodies and protect us from disease. Some bacteria, however, can cause disease—these bacteria are known as pathogens.
In order to minimize the risk of disease from bacteria, it is necessary to control their growth in a variety of ways, including the use of antibiotics, disinfectants, and antiseptics.
Under the proper conditions, bacteria can be grown in the laboratory. In this lab, you will analyze the effects of various antimicrobial treatments on the growth of three different bacteria— , , and.
Staphylococcus aurenus Haemophilusinfluenzae Streptococcus pneumoniae
The procedure below outlines the steps needed to grow bacteria and apply various antimicrobial treatments in the lab. Navigate through this virtual lab by selecting the arrows on the slider. Images and a description of each step are provided for you to follow along.
Three separate petri dishes containing agar will be inoculated with three different types of bacteria. Various antimicrobial agents will then be added to each dish. It is your job to measure the bacterial growth around each antimicrobial agent to determine the effectiveness of each treatment.
Procedure
Inoculate the agar in the petri dish with bacteria from one of the test tubes.
Begin with test tube #1.
Testtube #1: Staphylococcus aurenus
Testtube #2: Haemophilusinfluenzae
Testtube #3: Streptococcus pneumoniae
Dip a sterile metal loop into the sample and streak it onto the agar plate in a
zig-zag pattern.
Questions
1. Define antibiotic, antiseptic, and disinfectant. What is the main difference between the three types of microbial agents? Classify the seven treatments in this lab as either an antibiotic, antiseptic, or disinfectant.
2. Use the results from your data table to create a clustered bar graph. Plot the type of antimicrobial agent on the x-axis, and the size of bacterial growth inhibition (in mm) on the y-axis. Use a legend to differentiate between bacteria.
3. Compare the bars on your graph to determine the effectiveness of various antimicrobial agents on different bacteria. Which agents were effective at stopping growth of all of the bacteria? Which agents were bacteria specific? How do you know?
4. Describe the disease(s) caused by each of the three bacteria in this lab.
5. If you were a doctor treating a patient with pneumonia, which antibiotic would you prescribe? Why?
6. Why is it important for antibacterial drugs to be bacteria-specific?
Assessment Details
Your submission should include the following:
? Your answers to the questions written in your own words
All in-text citations and a reference list according to the APA style guide for sources used in your responses
?
Upload your complete answers to the
Dropbox.
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