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October 26, 2022

Yourclinical collaborator sharesan immortalized cell line that was derivedfrom a patient with leukemia.

Yourclinical collaborator sharesan immortalized cell line that was derivedfrom a patient with leukemia. While culturing this cell line you find that it spontaneously differentiates. Your flow cytometry experiment with antibodies against two cell surface markers CD34 and CD14 gives the result shown in the scatter plot. From this result you infer that the original leukemia cells were likely CD34+ and have the capacity to differentiate into CD14+ monocytes.

1. What is the immunophenotype of the cells in the four quadrants of the scatter plot?

2. Can you test the lineage of cells in Q4? List the potential surface markers you will test for and draw scatter plots with gates to show your strategy for data analysis.

3. Design an experiment to confirm that the CD34+ cells in Q1 have stem-like properties.

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