Aspartate has an R-group of -CH2-COOH with pKa1 = 2.10, pKa2 = 9.82 and pKaR = 3.86. Cysteine has an R-group of -CH2-SH with pKa1 = 2.05, pKa2 = 10.25 and pKaR = 8.00.

Aspartate has an R-group of -CH2-COOH with pKa1 = 2.10, pKa2 = 9.82 and pKaR = 3.86. Cysteine has an R-group of -CH2-SH with pKa1 = 2.05, pKa2 = 10.25 and pKaR = 8.00. From the pKas listed above, determine the pI for the molecule (remember, pKa1is the α-carboxy group and pKa2 is the α-amino group).

It might help you to draw an oligopeptide below of Asp-Cys showing the ionizable groups in their isoelectric state (that is, the total charge on the oligo = 0).

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