Collin County Community College Osmosis and Diffusion Bio Lab
Collin County Community College Osmosis and Diffusion Bio Lab
Collin County Community College Osmosis and Diffusion Bio Lab
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OSMOSIS AND DIFFUSION REPORT SHEET
Download the report sheet, complete it, and then submit it via Blackboard email as an attachment.Do not change the format.
All blue shaded areas require answers.
- Osmosis
Experiment (dry lab)
There are 3 diagrams of animal cells (a, b, c) and 3 diagrams of plant cells (a, b, c) in solutions of varying tonicity. Label the diagrams as isotonic solution, hypertonic solution, or hypotonic solution.
| Animal cells: | |
| (a) | |
| (b) | |
| (c) | |
| Plant cells: | |
| (a) | |
| (b) | |
| ( c) |
- Effect of temperature and size
- Effect of solute concentration
- Differential permeability of membranes
- Diffusion and osmosis in living cells
Observe the agar plate at 25°C, 30 min.
Experiment (dry lab)
| Potassium permanganate at 25°C | Janus Green at 25°C | ||
| Time | Distance in mm | Time | Distance in mm |
| 30 min | 30 min | ||
Potassium permanganate (294 Daltons) and Janus Green (511 Daltons) dyes
| Which dye has greater diffusion, potassium permanganate or Janus Green? | |
| Why? |
Observe the agar plate at 5°C, 30 min.
Experiment (dry lab)
| Potassium permanganate at 5°C | Janus Green at 5°C | ||
| Time | Distance in mm | Time | Distance in mm |
| 30 min | 30 min | ||
Potassium permanganate (294 Daltons) and Janus Green (511 Daltons) dyes
| Which dye has greater diffusion potassium permanganate or Janus Green? | |
| Why? |
Compare the agar plates at 5°C and 25°C.
Experiment (dry lab)
| Which agar plate shows greater diffusion, 5°C or 25°C? | |
| Why? |
Effect of temperature on diffusion of iodine
Experiment (at home lab)
QUESTIONS
| Temperature of your cups: | ||
| Is the food coloring evenly distributed in each? | ||
| Explain. | ||
Experiment (dry lab)
In the weight change column, record if the bag should increase in weight, decrease in weight, or stay about the same.
| # | Bag contents | Beaker contents | Weight change |
| 1 | 10 ml Distilled water | 200 ml Distilled water | |
| 2 | 10 ml 10% sucrose | 200 ml Distilled water | |
| 3 | 10 ml 20% sucrose | 200 ml Distilled water | |
| 4 | 10 ml 40% sucrose | 200 ml Distilled water | |
| 5 | 10 ml Distilled water | 200 ml 40% sucrose | |
| Which bag should gain the most weight? | |||
Experiment (dry lab)
| Test for | Is test positive or negative? |
| Starch | |
| Protein | |
| Chloride ion | |
| Sulfate ion |
| Which ones (starch, protein, chloride ions, sulfate ions) moved through the dialysis tubing and into the beaker? | |
| Why did some not move from the bag into the beaker? |
Experiment (dry lab)
A.Osmosis with red blood cells
| Describe the blood cells. | |
| 0.9% NaCl , Normal blood cells | |
| 10% NaCl, Crenated cells | |
| 1. What solution causes cells to appear this way?
Isotonic, hypertonic or hypotonic solution. |
2. What solution causes cells to appear this way?
Isotonic, hypertonic or hypotonic solution. |
- Osmosis with plant cells
| Describe the plant cells. | |
| 0% NaCl, Turgid cells | |
| 20% NaCl, Plasmolyzed cells | |
| 1. What solution causes cells to appear this way?
Hypertonic or hypotonic solution. |
2. What solution causes cells to appear this way?
Hypertonic or hypotonic solution. |
Diffusion in cucumber cells
Experiment (at home lab)
QUESTIONS
| 1. When comparing the firmness of the cucumber slices, how would you describe each, firm (turgid cells) or soft (plasmolyzed cells)? | |
| Cup 1 | |
| Cup 2 | |
| Cup 3 | |
| 2. What caused one to be firm and the other to be soft?
Explain. |
End of Lab J
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