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October 4, 2022

DNA Extraction

DNA Extraction Worksheet

1) What are the physical and chemical barriers in purifying the DNA from intact animal and plant cells?

2) What is the significance of using chelating agents, such as InstaGene, in DNA purification?

3) What structures must be broken to release the DNA from the cells? What molecules may still be bound with the DNA after extraction?

4) Why is DNA insoluble in ethanol?

5) Other than detergent what other possible ingredients are added to the cell lysis solution?

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