Identify the major similarities and differences between prokaryotic and eukaryotic cells.
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Pre-Lab Questions:
1. Identify the major similarities and differences between prokaryotic and eukaryotic cells.
2. Where is the DNA housed in a prokaryotic cell? Where is it housed in a eukaryoticcell?
3. Name three structures which provide support and protection in a eukaryotic cell.
Experiment 1: Cell Structure and Function
Post-Lab Questions
Labeleachofthearrowsinthefollowingslideimages:
A-
B-
Onion root tip, 1000x.
Whatisthedifferencebetweentheroughandsmoothendoplasmicreticulum?
3. Would an animal cell be able to survive without a mitochondria? Why or whynot?
4. What is the function of alysosome?
Experiment 2: Exploring Cell Size
Record the cell radius and time to center of the cell in Table 2. Then, calculate the surface area, volume, and surface area:volume ratio (equations provided below).
Table 2: Surface Area and Volume in Relation to Cell Size
Radius (µm)
Surface Area (µm2)
Volume (µm3)
Surface Area:Volume Ratio
Time to Center of the Cell (s)
15
2,827.4
13,783.74
1:4875
0.075
30
11,309.7
110,269.9
1:9.75
0.15
45
25,446.9
372,160.92
1:14.625
0.225
60
45,238.9
882,159.22
1:19.5
0.3
75
70,685.8
1,722,967.2
1:24.38
0.375
90
101,787.6
2,977,287.36
1:29.25
0.45
105
138,544.2
4,727,822,1
1:34.13
0.525
120
180,955.7
7,057,273.74
1:50.94
0.6
135
229,022.1
10,048,344.8
1:43.88
0.675
150
2822,743.3
13,783,737.77
1:48.75
0.75
Post-Lab Questions
1. Createagraphofthetimetothecenterofthecellasafunctionofthesurfacearea:volume ratio.
2. Howdidthesurfaceareaaffectthediffusionofthespheresorblocksrepresentingasimple cell? What about the volume? What about the surface area to volume ratio? Which of these had the greatest effect on the diffusion of the spheres orblocks?
3. How does this experiment demonstrate the need for larger cells to divide?
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