Below is a list of the resources that you need t
Exercise 7B Postlab
Instructions:
- Below is a list of the resources that you need to complete the Postlab Assignment for Exercise 7B.
- The correctness and effort of your work determines your grade on this set of activities.
- This assignment should be completed individually. Any assignments that have been duplicated or plagiarized (in part or in total) will not be accepted. Because many of you may not have experience with these types of activities, a grading rubric exists for you to use.
Powerpoint Slides: In this part of the Postlab assignment, you continue to build your final presentation for the semester-long Wolbachia project.
- Your final presentation for this course will include these slides that cover this information.
- Refer to the Powerpoint "Style Guide" to earn a better grade!
Slide 1: Create a slide that contains an appropriate, descriptive title and a table that summarizes the expected results from your PCR activity.
- Include an appropriate, descriptive title.
- Refer to Table 6-4 of Exercise 6 in your lab manual as a guide for what to include (page 207).
- DO NOT just copy Table 6-4.
- DO NOT include all of the information from the table. Evaluate which information in Table 6-4 is appropriate for this part of the assignment. Any unnecessary information that is included will result in a deduction.
- Use specimen IDs (do not use generic terms such as sample/unknown #1, #2, etc).
- Don't include "?" as expected results for Wolbachia for your insect specimen. You made a prediction based on the literature. What do you expect based on your prediction?
Slide 2: Create a slide that contains a labelled figure of your gel that you began creating in Part five of Exercise 7B in lab (directions on page 260). To do this, take your gel picture and edit it in Powerpoint to include the following information on the slide for full credit:
- an appropriate, descriptive title (Hint: Again, “Gel Electrophoresis” is NOT informative!)
- an enlarged, cropped view of the original gel image, which should fill most of the slide
- labels for gel lanes, ladder band sizes (including units), insect and Wolbachia gene names and true sizes (including units)
- type of gel (2% of agarose) and voltage (100V for 45 minutes) used for electrophoresis.
Slide 3: Create a slide that includes the prediction, the evaluation of the prediction, and the relevance of the data to what is already known.
- Include an appropriate, descriptive title.
- Answer the question, "Did your data support your prediction or not?"
- Compare your results to the information that you found about Wolbachia infection in your insect Order in the peer-reviewed literature and in the local collection data (from Exercise 3).
Slide 4: Create a slide that includes a detailed reflection that describes one benefit of your individual experience in this lab course.
- Include an appropriate, descriptive title.
- This is your opportunity to provide a thoughtful, unique reflection on your experience working through the Wolbachia project in this course.
Assignment Submission Instructions
Save and name your file
- Report your work as information in 4 MS Powerpoint slides. Details on what each of these slides should include can be found above.
- Check yourself using the associated rubric (Exercise 7B Postlab Assignment" Folio Rubric).
- You may NOT submit Google Slides or any other software equivalent. If you prepare your slides in Google Slides, be sure to download as a MS Powerpoint file before uploading to the postlab dropbox.
- Save your assignment in the appropriate file format as "Exercise7BPostlab" followed by your three initials. (For example, "Exercise7BPostlabGJD.pptx")
- Upload and Submit your MS Powerpoint file to this dropbox (Exercise 7B Postlab Assignment Folio Dropbox Folder).
3/27/22, 2:51 AM Preview Rubric: Exercise 7B Postlab Assignment Rubric – Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2) – Georgia …
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Exercise 7B Postlab Assignment Rubric Course: Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2)
PCR Acceptable Developing Developing Developing Absent Criterion Score
Control and
Sample ID
Names
/ 3
Expected/Pr
edicted
Results
/ 10
Relevance
of
information
/ 5
3 points
All control and
sample ID
names are
included.
2 points
One sample or
control or
sample ID
name is
missing OR
sample names
are are not
properly listed
(e.g.
MEH12F18-
1).
1 point
More than one
control or
sample ID
name is
missing.
0 points 0 points
No control
names or
sample IDs are
included.
10 points
The
expected/pred
icted results
for all PCR
samples are
listed
correctly.
8 points
The
expected/pred
icted results
for ONE
control or
unknown PCR
sample is not
listed
corrected.
6 points
The
expected/pred
icted results
for TWO
control or
unknown PCR
samples are
not listed
corrected.
4 points
The
expected/pred
icted results
for MORE
THAN TWO
control or
unknown PCR
samples are
not listed
corrected.
0 points
No
expected/pred
icted results
are presented
correctly, or
the PCR slide
was not
included in the
student's
submission.
5 points
All tabular
data are
relevant. No
unnecessary
information is
presented.
4 points
The table
includes ONE
additional
irrelevant item
(e.g. amounts
of water,
primers,
template, etc)
3 points
The table
includes TWO
additional
irrelevant item
(e.g. amounts
of water,
primers,
template, etc)
1 point
The table
includes
THREE or
more
additional
irrelevant item
(e.g. amounts
of water,
primers,
template, etc)
0 points
There was no
PCR slide
included in the
student's
submission.
3/27/22, 2:51 AM Preview Rubric: Exercise 7B Postlab Assignment Rubric – Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2) – Georgia …
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Gel
Electroph
oresis
Slide
Acceptable Developing Developing Developing Developing Absent Criterion Score
Lane
Labeling
/ 10
Ladder
Band
Labeling
/ 5
PCR
Product
Bands
/ 16
10 points
All lanes are
labeled
appropriatel
y.
8 points
One lane is
not labeled
appropriatel
y.
6 points
Two lanes
are not
labeled
appropriatel
y.
4 points
Three lanes
are not
labeled
appropriatel
y.
2 points
Four or more
lanes are not
labeled
appropriatel
y.
0 points
No lanes are
labeled.
5 points
The bands in
the DNA
ladder are
labeled
appropriatel
y.
4 points
The bands in
the DNA
ladder are
labeled but
at least ONE
of the labels
is not
appropriate
OR cannot
be easily
read (too
small or
crowded)
OR no units
are included.
2 points
The bands in
the DNA
ladder are
labeled but
at TWO-
THREE of
the labels is
not
appropriate
OR cannot
be easily
read (too
small or
crowded)
OR no units
are included.
1 point
The bands in
the DNA
ladder are
labeled but
at FOUR of
the labels
are not
appropriate
OR cannot
be easily
read (too
small or
crowded)
OR no units
are included.
0 points 0 points
No labeling
of the DNA
ladder is
presented.
16 points
Insect CO1
and
Wolbachia 16S rRNA
bands are
labeled
appropriatel
y.
12 points
At least
ONE-TWO
of the
following is
missing OR
labeled
incorrectly:
Insect CO1
label, 708bp,
Wolbachia 16S rRNA
label, 438bp.
8 points
THREE-
FOUR of the
following is
missing OR
labeled
incorrectly:
Insect CO1
label, 708bp,
Wolbachia
16S rRNA
label, 438bp.
4 points
MORE
THAN FOUR
of the
following is
missing OR
labeled
incorrectly:
Insect CO1
label, 708bp,
Wolbachia
16S rRNA
label, 438bp.
0 points 0 points
No labels on
the Insect
CO1 or
Wolbachia
16S rRNA
PCR
products are
presented.
3/27/22, 2:51 AM Preview Rubric: Exercise 7B Postlab Assignment Rubric – Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2) – Georgia …
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Gel
Electroph
oresis
Slide
Acceptable Developing Developing Developing Developing Absent Criterion Score
Agarose
and
Voltage
/ 44 points
Gel
electrophore
sis
conditions
are
accurately
presented.
2 points
Either the
percentage
of agarose in
the gel OR
the voltage
is not
presented
accurately or
is missing.
0 points 0 points 0 points 0 points
Neither the
percentage
of agarose in
the gel nor
the voltage
is presented
OR both are
inaccurately
presented.
Conclusions/R
eflections Acceptable Developing Developing Absent Criterion Score
Evaluation of
Prediction
/ 66 points
Presentation
clearly and
correctly
describes how
the results
support or do
not support the
prediction.
4 points
Student data are
not clearly linked
to the prediction,
and/or student
did not clearly
describe whether
the prediction
was supported or
not.
2 points
Student data are
not linked to the
prediction,
and/or student
did not clearly
describe whether
the prediction
was supported or
not.
0 points
Student did not
evaluate the
prediction after
completing the
experiment.
3/27/22, 2:51 AM Preview Rubric: Exercise 7B Postlab Assignment Rubric – Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2) – Georgia …
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Conclusions/R
eflections Acceptable Developing Developing Absent Criterion Score
Relevance of
Conclusions
/ 6
Reflection / 3
6 points
Relevance of
conclusions is
discussed as it
relates to
information
obtained from
peer-reviewed
scientific journal
articles and the
local collection
data.
4 points
An attempt is
made to discuss
the relevance of
the conclusions
as they relate to
data obtained
from peer-
reviewed
scientific journal
articles OR the
local collection
data.
2 points
An attempt is
made to discuss
the relevance of
the conclusions
as they relate to
data obtained
from peer-
reviewed
scientific journal
articles OR the
local collection
data but needs
significant
improvement.
0 points
Relevance of the
conclusions was
not discussed.
3 points
A thoughtful,
unique reflection
is presented in
detail.
2 points
A reflection is
presented but is
somewhat
detailed, unique,
or thoughtful.
1 point
A reflection is
presented but is
not detailed,
unique, or
thoughtful.
0 points
No reflection is
presented.
Presentation
Appearance Acceptable Developing Absent Criterion Score
Slide Titles / 2 2 points
All slides have
appropriate,
descriptive titles.
1 point
One slide title is
missing OR One
slide title is
inappropriate.
0 points
Both slide titles are
missing OR Both
slide titles are
inappropriate OR
One slide title is
missing and the
other title is
inappropriate OR No
slide titles are
present.
3/27/22, 2:51 AM Preview Rubric: Exercise 7B Postlab Assignment Rubric – Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2) – Georgia …
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Presentation
Appearance Acceptable Developing Absent Criterion Score
Information Flow / 2
Amount of Text / 2
Font Size and
Style
/ 2
Spelling and
Grammar
/ 2
Image
Appearance
(Expected
Results)
/ 2
2 points
Information in the
slides is organized
and clearly relays
the expected or
actual results.
1 point
Information in the
slides is not well
organized or there is
additional
distracting
information included
(e.g., unnecessary
arrows, lines, or
animations)
0 points
Information in the
slides was poorly
organized and
unclear.
2 points
Appropriate amount
of text was included
on the slides.
1 point
Too much or too
little text was
included on ONE
slide.
0 points
Too much or too
little text was
included on BOTH
slides.
2 points
Font size is
appropriate. Font
style is consistent.
1 point
Slides show minor
issues with Fonts:
Font size is too
large, too small, or
inconsistent.
0 points
Font size is too
large, too small, or
inconsistent in
multiple areas of
slides.
2 points
No spelling or
grammar errors
exist.
1 point
Some spelling or
grammar problems
exist.
0 points
Many problems with
spelling and/or
grammar exist.
2 points
Expected results are
presented in tabular
format AND Table is
created using
PowerPoint
software.
1 point
Results are
presented but are
not in tabular format
OR Screenshot of
table from the lab
manual is inserted.
0 points
No expected results
are presented.
3/27/22, 2:51 AM Preview Rubric: Exercise 7B Postlab Assignment Rubric – Spring 2022 – Principles of Biology I Lab (BIOL-1107L-OF2) – Georgia …
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Total / 84
Overall Score
Presentation
Appearance Acceptable Developing Absent Criterion Score
Image
Appearance (Gel
Results)
/ 2
Scientific Names / 2
2 points
Gel Image is a good
size, appropriately
cropped, focused
and not distorted.
1 point
The gel image had a
problem with ONE
of the following: not
the appropriate size,
not cropped
appropriately, not
clear (was
distorted).
0 points
The gel image had a
problem with MORE
THAN ONE of the
following: not the
appropriate size, not
cropped
appropriately, not
clear (was distorted)
OR Gel image was
not included.
2 points
Scientific names are
written correctly.
1 point
Scientific names are
written incorrectly.
0 points
No scientific names
are included.
A 75.6 points
minimum
B 67.2 points
minimum
C 58.8 points
minimum
D 50.4 points
minimum
F 0 points
minimum
,
Exercise 7B Postlab
Instructions:
· Below is a list of the resources that you need to complete the Postlab Assignment for Exercise 7B.
· The correctness and effort of your work determines your grade on this set of activities.
· This assignment should be completed individually. Any assignments that have been duplicated or plagiarized (in part or in total) will not be accepted. Because many of you may not have experience with these types of activities, a grading rubric exists for you to use.
Powerpoint Slides: In this part of the Postlab assignment, you continue to build your final presentation for the semester-long Wolbachia project.
· Your final presentation for this course will include these slides that cover this information.
· Refer to the Powerpoint "Style Guide" to earn a better grade!
Slide 1: Create a slide that contains an appropriate, descriptive title and a table that summarizes the expected results from your PCR activity.
· Include an appropriate, descriptive title.
· Refer to Table 6-4 of Exercise 6 in your lab manual as a guide for what to include (page 207).
· DO NOT just copy Table 6-4.
· DO NOT include all of the information from the table. Evaluate which information in Table 6-4 is appropriate for this part of the assignment. Any unnecessary information that is included will result in a deduction.
· Use specimen IDs (do not use generic terms such as sample/unknown #1, #2, etc).
· Don't include "?" as expected results for Wolbachia for your insect specimen. You made a prediction based on the literature. What do you expect based on your prediction?
Slide 2: Create a slide that contains a labelled figure of your gel that you began creating in Part five of Exercise 7B in lab (directions on page 260). To do this, take your gel picture and edit it in Powerpoint to include the following information on the slide for full credit:
· an appropriate, descriptive title (Hint: Again, “Gel Electrophoresis” is NOT informative!)
· an enlarged, cropped view of the original gel image, which should fill most of the slide
· labels for gel lanes, ladder band sizes (including units), insect and Wolbachia gene names and true sizes (including units)
· type of gel (2% of agarose) and voltage (100V for 45 minutes) used for electrophoresis.
Slide 3: Create a slide that includes the prediction, the evaluation of the prediction, and the relevance of the data to what is already known.
· Include an appropriate, descriptive title.
· Answer the question, "Did your data support your prediction or not?"
· Compare your results to the information that you found about Wolbachia infection in your insect Order in the peer-reviewed literature and in the local collection data (from Exercise 3).
Slide 4: Create a slide that includes a detailed reflection that describes one benefit of your individual experience in this lab course.
· Include an appropriate, descriptive title.
· This is your opportunity to provide a thoughtful, unique reflection on your experience working through the Wolbachia project in this course.
Assignment Submission Instructions
Save and name your file
1. Report your work as information in 4 MS Powerpoint slides. Details on what each of these slides should include can be found above.
2. Check yourself using the associated rubric ( Exercise 7B Postlab Assignment" Folio Rubric ).
3. You may NOT submit Google Slides or any other software equivalent. If you prepare your slides in Google Slides, be sure to download as a MS Powerpoint file before uploading to the postlab dropbox.
4. Save your assignment in the appropriate file format as "Exercise7BPostlab" followed by your three initials. (For example, "Exercise7BPostlabGJD.pptx")
5. Upload and Submit your MS Powerpoint file to this dropbox ( Exercise 7B Postlab Assignment Folio Dropbox Folder ).
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