Activity – Enzymes
Data Sheet: Activity – Enzymes
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Name |
Course |
Date |
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|
BIOL 103 |
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Activity Data Code FORMTEXT
FFFFFFFF00000000140006005400650078007400340035000000000000000000000000000000000000000000000000000000
Procedure I – Enzyme Reaction Rate – Concentration Dependence
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
|
Trial |
Concentration Level |
Oxygen Concentration Change Data (ppt) |
Elapsed Time (s) |
Enzyme Reaction Rate (ppt/s) |
|
1 |
2.5% |
|
20.0 |
|
|
2 |
5.0% |
|
20.0 |
|
|
3 |
10% |
|
20.0 |
|
Observations and Questions
[1] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.
[2] Convert enzyme concentrations of 2.5%, 5.0%, and 10% to ppt units.
[3] For Procedure I/Table I, identify:
Independent variable:
Dependent variable:
Control variables:
[4] Draw a graph of the independent variable on the x-axis (abscissa) and the dependent variable on the y-axis (ordinate). Label the axis titles and units.
Procedure II – Enzyme Reaction Rate – Temperature Dependence
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
|
Trial |
Temperature (°C) |
Oxygen Concentration Change Data (ppt) |
Elapsed Time (s) |
Enzyme Reaction Rate (ppt/s) |
|
1 |
20.0 |
|
20.0 |
|
|
2 |
30.0 |
|
20.0 |
|
|
3 |
55.0 |
|
20.0 |
|
Observations and Questions
[5] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.
[6] Using the data you reported in the Table above, describe the effect of temperature on the reaction rate.
Procedure III – Enzyme Reaction Rate – pH Dependence
Complete the table below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
|
Trial |
pH Level |
Oxygen Concentration Change Data (ppt) |
Elapsed Time (s) |
Enzyme Reaction Rate (ppt/s) |
|
1 |
6.0 |
|
20.0 |
|
|
2 |
7.0 |
|
20.0 |
|
|
3 |
9.0 |
|
20.0 |
|
Observations and Questions
[7] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.
[8] Choose either a bar graph or a line graph format and plot the data for Procedures II and III. Plot the data for each Procedure on a separate plot.
[9] Compare the plot of the data for Procedures I, II, and III. Describe the differences among the three plots.
[10] What conclusions can draw about the effects of concentration, temperature and pH on the reaction rate after comparing the plots from the three different Procedures?
[11] Choose one of the factors that impacts reaction rate (concentration, temperature, or pH) and, in your own words, provide a plausible biological explanation for your experimental results for that factor.
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