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60 mL Sterile phosphate buffered saline (PBS)
250 mL Beaker
Hot pad
10 Disposable sterile transfer pipette
8 Disposable sterile plate spreader
(6) 5 cm. Petri dishes
Nutrient agar
Parafilm™
1 Pair of gloves
10 mL Graduated Cylinder
Permanent marker
*Microwave or other boiling water bath
*1 tsp. Soil sample
*10% Bleach solution
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