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December 18, 2020

Analysis of the effects of a range of drug molecules on evoked contractions of the guinea pig ileum

Analysis of the effects of a range of drug molecules on evoked contractions of the guinea pig ileum

PY162 PAIN CASE LABORATORY EXERCISE
Demonstration to investigate some sites of drug action on gastrointestinal tract motility.
Objectives
Understand the requirements for maintaining the functioning of ex vivo tissue.

Understand the pharmacological regulation of G I motility

Understand the pharmacological and physiological regulation of autonomic control of the gut.

Appreciate the rationale for the uses of drugs that regulate gut function in the treatment of disease.
Introduction
Pharmacists are the experts in the healthcare team on medicines and it is essential that they understand the how drug molecules affect the function of different organ systems in the body. The aim of this demonstration is to illustrate the functional role of different drugs on gastrointestinal tract motility using a section of guinea pig colon. You will examine the effects of a variety of drugs e.g. morphine or naloxone on the contractions of the guinea pig ileum evoked by trans-mural stimulation. Sections of the gastrointestinal tract can be stimulated by passing a current across the muscular wall of the tissue (trans-mural stimulation). A diagram of the structure of the gastrointestinal tract is shown below.

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