We need to discover the precise relationship between absorbance at 595nm in the Bradford Assay and concentration of protein in our sample. We need to generate a standard curve that descri
We need to discover the precise relationship between absorbance at 595nm in the Bradford Assay and concentration of protein in our sample. We need to generate a standard curve that describes this relationship. The best-fit line on this curve will allow us to convert the absorbance readings that we find for our milk samples into amounts of protein.
1. Obtain seven plastic microcentrifuge tubes and label their lids 1-7 with a sharpie pen.
2. You will be provided with a stock solution of BSA that is at a concentration of 100 (microgram) ug/mL. Each one of your
seven microfuge tubes needs to get a different amount of BSA (as shown below). Calculate the volume of
stock BSA solution to be added to each tube and then add that amount of BSA.
1. no BSA (blank)
2. 0.3 ug BSA
3. 1 ug BSA
4. 3 ug BSA
5. 6 ug BSA
6. 10 ug BSA
7. 25 ug BSA
So, you have a solution of BSA that is 100 ug/ml. The key part of the
question is, what volume of this solution would contain 0.3 ug of BSA?
(or 1 ug, 3 ug, 6 ug… for the other tubes.)
There are a number of ways you could approach this. The
easiest way is to realize that 100 ug/ml means that 1 ml contains 100 ug
of BSA. Then, what volume would contain only 0.3 ug of BSA?
Once you get the volumes of BSA you need, remember that you want the
FINAL volume of each tube to be 600 ul. So, don’t forget to determine
how much water you’re going to need to add to your BSA to get there.
Collepals.com Plagiarism Free Papers
Are you looking for custom essay writing service or even dissertation writing services? Just request for our write my paper service, and we'll match you with the best essay writer in your subject! With an exceptional team of professional academic experts in a wide range of subjects, we can guarantee you an unrivaled quality of custom-written papers.
Why Hire Collepals.com writers to do your paper?
Quality- We are experienced and have access to ample research materials.
We write plagiarism Free Content
Confidential- We never share or sell your personal information to third parties.
Support-Chat with us today! We are always waiting to answer all your questions.
![](https://collepals.com/wp-content/plugins/posts-import/files/order-now-with-paypal.png)