Determination of the paracetamol and caffeine content of solutions
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Get it written →1. What is it about the molecular structures of paracetamol and caffeine that makes separation by TLC possible?
2. What is the fallibility of using both a low sample number and using a hand drawn calibration plot technique for obtaining the best results? Compare your results by using a hand drawn graph and one using a computer with a regression line.
3. What would you see in both TLC and quantification by UV spectroscopy if the paracetamol degraded whilst in solution?
4. What could have contributed to any errors made?
5. What parameters are likely to have influenced the solubility of both paracetamol and caffeine in TLC work and crushed paracetamol tablet powder in the assay work? What activities could be used to maximise solubilisation of drug in the analytical samples?
6. What other analytical chemistry techniques accessible in the University laboratory could be used to aid identification of the compounds?
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